Using the glycan microarray approach, natural carbohydrate ligands were established, and the glycotope for GYL was reconstructed as “Galβ1–4GlcNAcβ obligatory containing an additional fragment”, like a sulfate group or a methyl group of fucose or N-acetylgalactosamine residues.īivalves are a group of marine fauna vulnerable to various pollutants and aquatic microorganisms as they are sedentary filtering organisms. This fact indicates the presence of a multigene family of GYL-like C-type lectins in the bivalve G. Additionally, when confirming the GYL sequence, eight isoforms of this lectin were identified. The potential CRD tertiary structure of the GYL adopted CTL-typical long-form double-loop structure and included three disulfide bridges at the bases of the loops. N-glycans are made up of xylose, mannose, D-glucosamine, 3-O-methylated galactose, D-quinovoses, and 3-O-methylated 6-deoxy-D-glucose. GYL is a glycoprotein containing two N-glycosylation sites per subunit. The deduced amino acid sequence showed similarity to other CTLs. Here, we describe the cDNA cloning of lectin from the bivalve Glycymeris yessoensis (GYL), which encodes 161 amino acids and the C-type carbohydrate recognition domain (CRD) with EPN and WND motifs. C-type lectins (CTLs) are a family of carbohydrate-binding proteins that mediate multiple biological events, including adhesion between cells, the turnover of serum glycoproteins, and innate immune system reactions to prospective invaders.
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